Massive edema of ovary with cytogenetic alteration of chromosome 12q13-15 in adolescent patient: a case report
© De Cecio et al.; licensee BioMed Central Ltd. 2013
Received: 14 January 2013
Accepted: 1 February 2013
Published: 7 February 2013
The massive edema of ovary, with or without fibromatosis, is a rare tumor-like entity characterized by an increase in volume of one or both ovaries for accumulation of edema fluid in the stroma that separates the follicular structures.
We report a rare case, very peculiar also for its association with a massive stromal fibromatosis and for the presence, never described, of tumoral areas with CHOP gene translocation, on chromosome 12q13–15.
KeywordsOvarian edema Sarcoma-like CHOP translocation
The massive ovarian edema is a rare benign lesions mimicking neoplastic lesions and typically affects young women more frequently in the second and third decades of life.
Edema involves one or both ovaries and its etiology is related to partial and intermittent ovarian pedicle torsion that interfere with lymphatic drainage. The edema typically presents with abdominal pain less frequently with menstrual disorders .
Androgenic manifestations (virilization, hirsutism ect.) are described among the symptoms, due to the presence of luteinizzate stromal cells that are found in 30/40% of cases . An abdominal mass ranging in size from 5 cm to about 35 cm can be appreciated on palpation.
A 15-year-old girl has been admitted to gynecological surgery of our Institute for the presence of suspect ovarian masses. During surgery, frozen sections excluded malignancy.
The sample had been submitted to RT-PCR analysis, after RNA purification, to verify the presence of the fusion transcripts CHOP/FUS and CHOP/EWS [3, 4], typically encountered of myxoid liposarcoma. However, the molecular analysis did not reveal the presence of these transcripts.
In case that we have described, in addition to massive edema, the presence of a significant stromal fibromatosis was observed. Both aspects seem to be the final event of the same disorder (torsion of the pedicle and primitive stromal proliferation).
Moreover, in fibromatosis area follicles are surrounded by stromal myofibroblastic proliferation . Before the confirmation of this diagnosis, it is important exclude other ovarian diseases. The differential diagnosis should be made with sclerosing stromal tumor, the thecoma luteinized with sclerosing peritonitis and the ovarian myxoma .
The sclerosing stromal tumor has a characteristic pseudo-lobular pattern with focal hyalinization and compression of the surrounding ovarian tissue. Differently from edema and fibromatosis this cancer does not spare the follicles, the tumor involves only one ovary and the age of onset is most frequently around 27 years. The immunohistochemical profile is characterized by a positivity of stromal cells for Vimentin, calretinin, alpha-inhibin and CD34.
The myxoma is a benign tumor of the ovary characterized by a myxoid stroma in which stellate cells and a vascular component consists of small capillaries are immersed; the age most frequently affected is between 16 and 45 years and is usually unilateral.
The cells are positive for Vimentin and muscle specific actin.
The thecoma luteinized with sclerosing peritonitis consists of spindle cells mixed with luteinized cells with diffuse edema with many mitoses and in 30% of cases occur in women under 30 years. The neoplastic cells were positive for alpha-inhibin and calretinin. These lesions have in common the change of follicles that are not recognizable.
The morphological analysis and immunohistochemical staining of our case has confirmed the diagnosis of massive edema of the ovary with fibromatosis, however, the sample also showed suspicious myxoid-like areas. For this reason, we decided to realize a cytogenetic analysis typical of myxoid liposarcoma to detect the CHOP gene translocation.
In the literature only one case of ovarian myxoid liposarcoma with this chromosomal aberration in a young woman has been described . However, the presentation of the lesion is extremely heterogeneous and being present areas with translocated cells. Furthermore, the analysis of RT-PCR performed to identify the fusion transcripts CHOP/FUS and CHOP/EWS [7, 8] was negative. Therefore, we exclude that this is a rare case of myxoid liposarcoma of ovary.
Our data can suggest the existence of a different fusion partner for CHOP, but, obviously, more detailed molecular studies are required to support our hypothesis.
For the pathologist and the clinician is important to know this benign disease that for its presentation may mimic a neoplastic pathology. In fact, the massive edema of ovary generally occurs in young women where the reproductive capacity must be preserved, avoiding surgery demolition. Moreover, the development of a more adequate molecular characterization, is necessary not only for the differential diagnosis of this malignance.
Written informed consent was obtained from the patient for publication of this Case Report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
- Nogales FF, Martin-Sances L, Mendoza-Garcia E, Salamanca A, González-Nuñez MA, Pardo Mindán FJ: Massive ovarian oedema. Histopathology 1996, 28: 229–234. 10.1046/j.1365-2559.1996.d01-420.xPubMedView ArticleGoogle Scholar
- Siller BS, Gelder MS, Alvarez RD, Partridge EE: Massive edema of the ovary associated with androgenic manifestations. South Med J 1995, 88: 1153–1155. 10.1097/00007611-199511000-00015PubMedView ArticleGoogle Scholar
- Hisaoka M, Tsuji S, Morimitsu Y, Hashimoto H, Shimajiri S, Komiya S, Ushijima M: Detection of TLS/FUS-CHOP fusion transcripts in myxoid and round cell liposarcomas by nested reverse transcription-polymerase chain reaction using archival paraffin-embedded tissues. Diagn Mol Pathol 1998, 7: 96–101. 10.1097/00019606-199804000-00006PubMedView ArticleGoogle Scholar
- Matsui Y, Ueda T, Kubo T, Hasegawa T, Tomita Y, Okamoto M, Myoui A, Kakunaga S, Yasui N, Yoshikawa H: A novel type of EWS-CHOP fusion gene in myxoid liposarcoma. Biochem Biophys Res Commun 2006, 348: 437–440. 10.1016/j.bbrc.2006.07.085PubMedView ArticleGoogle Scholar
- Irving JA, McCluggage WG: Ovarian spindle cell lesions: a review with emphasis on recent developments and differential diagnosis. Adv Anat Pathol 2007, 14: 305–319. 10.1097/PAP.0b013e3180ca8a5bPubMedView ArticleGoogle Scholar
- Tirabosco R, Arora R, Ye H, Brunell C, Halai D, Flanagan AM: Primary myxoid liposarcoma of the ovary in an adolescent girl: a case report. Int J Gynecol Pathol 2010, 29: 256–259. 10.1097/PGP.0b013e3181c03fc9PubMedView ArticleGoogle Scholar
- Crozat A, Aman P, Mandahl N, Ron D: Fusion of CHOP to a novel RNA-binding protein in human myxoid liposarcoma. Nature 1993, 363: 640–644. 10.1038/363640a0PubMedView ArticleGoogle Scholar
- Panagopoulos I, Höglund M, Mertens F, Mandahl N, Mitelman F, Aman P: Fusion of the EWS and CHOP genes in myxoid liposarcoma. Oncogene 1996, 12: 489–494.PubMedGoogle Scholar
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