Relative expression of proprotein convertases in rat ovaries during pregnancy
© Kwok et al.; licensee BioMed Central Ltd. 2013
Received: 18 October 2013
Accepted: 6 December 2013
Published: 11 December 2013
Proprotein convertases are a family of serine proteinases that are related to bacterial subtilisin and yeast kexin. They are involved in posttranslational processing of the precursors of a vast number of cellular proteins. With the exception of PC1/3, the relative expression levels of the proprotein convertases in the ovary during pregnancy have not been reported. The purpose of this study is to determine by real-time PCR the relative expression levels of all nine proprotein convertases in rat ovaries during pregnancy and at 3 days postpartum.
RNA was extracted from ovaries at Day 0, 4, 9, 11, 13, 15, 18, and 20 of pregnancy as well as 3 days postpartum. Relative expression levels of Pcsk1, Pcsk2, Furin, Pcsk4, Pcsk5, Pcsk6, Pcsk7, Mbtps1 and Pcsk9 were determined with real-time PCR. Results were reported as fold-change over the level at Day 0 of pregnancy.
Results showed that Pcsk1 and Pcsk6 were upregulated as gestation advanced, in parallel with an observed increase in relaxin transcript. Pcsk2 showed downregulation as gestation advanced, while Pcsk5 showed relatively higher levels in early pregnancy and postpartum, but lower level in mid-pregnancy. On the other hand, Furin, Pcsk4, Pcsk7, Mbtps1 and Pcsk9 showed little change of expression throughout gestation.
PC1/3 (PCSK1) and PACE4 (PCSK6) may play an important role in proprotein processing in the ovary during late pregnancy.
KeywordsProhormone convertase Gene expression Posttranslational processing
Proprotein convertases are a family of serine proteinases that are related to bacterial subtilisin and yeast kexin. They are involved in posttranslational processing of a vast number of cellular proteins leading to their activation and sometimes inactivation. There are nine members identified so far: they are PC1/3, PC2, Furin, PC4, PC5/6, PACE4, PC7, SKI-1/S1P, and PCSK9 (NARC-1) . Their genes are named Pcsk1, Pcsk2, Furin, Pcsk4, Pcsk5, Pcsk6, Pcsk7, Mbtps1, and Pcsk9. The first seven members process precursor proteins at single or paired basic amino acids with the motif of (R/K)Xn(R/K)↓ (where R = arginine, K = lysine, X = any amino acid). They share a cleavage redundancy towards numerous substrates, such as protein hormones, receptors, adhesion molecules and metalloproteinases. The eighth member, SKI-1/S1P, cleaves membrane-bound transcription factors and N-acetylglucosamine-1-phosphotransferase at the motif of RX(L/V/I)X↓ (where L = leucine, V = valine, I = isoleucine), and the last member, PCSK9, cleaves itself at VFAQ152↓ (valine-phenylalanine-alanine-glutamine152) sequence .
Of these nine proprotein convertases, Furin, PC5/6, PACE4, PC7, and SKI-1/S1P are ubiquitously expressed [3–7]. Expression of PC1/3 and PC2 is restricted to neuroendocrine tissues . PC4 is expressed primarily in male germ cells, although it is also expressed in ovary and placenta . PCSK9 (NARC-1) is expressed in adult liver, small intestine and kidney . These proprotein convertases have been implicated to play an important role in follicle development and ovulation. PC5/6 was upregulated in rat ovarian follicles by gonadotropins and may be involved in the processing of precursors of transforming growth factor beta (TGFβ) and matrix metalloproteinases . Both PC5/6 and inhibin were upregulated during follicle development in mouse ovary, suggesting that PC5/6 may be involved in inhibin subunit processing . PACE4 (PCSK6) expression in preantral granulosa cells was upregulated by follicle-stimulating hormone (FSH), but was suppressed by factors secreted by full-grown oocytes from antral follicles . The change of PACE4 expression may suggest a change of mechanism involved in the processing of the precursors of the TGFβ family. Using Pcsk 6-knockout mice, Mujoomdar and co-workers  showed that PACE4 played an important role in maintaining normal cellular and tissue homeostasis in the ovary. Expression of Pcsk 6 in human granulosa cells and oocytes was suppressed by bone morphogenetic proteins, suggesting that it was subjected to bone morphogenetic protein negative feedback . Furin was upregulated in rat ovary treated with gonadotropin, whereas both matrix metalloproteinase 2 (MMP2) activation and oocyte release were decreased after treatment with furin inhibitor . These results suggest a role of furin in the breakdown of follicular wall during ovulation. Furin may also be involved in proliferation of granulosa cells, since knockdown of furin expression with furin siRNA decreased proliferation of granulosa cells . Expression of these proprotein convertases in the ovary during gestation has not been well studied. Our laboratory has cloned and characterized multiple PC1/3 transcripts from porcine ovary . In situ hybridization showed that PC1/3 and relaxin transcripts were colocalized in large luteal cells of porcine ovary and levels of both transcripts increased as gestation advanced . This suggests that PC1/3 may be involved in posttranslational processing of prorelaxin. The purpose of the present study is to investigate the relative expression levels of all nine proprotein convertases in rat ovaries during gestation using real-time PCR.
Animals and tissue preparation
Holtzman Sprague-Dawley rats were obtained from Harlan Laboratories (Indianapolis, IN). The animals were housed in an environmentally controlled facility with lights on from 0600 to 2000 hr and allowed free access to food and water. Timed pregnancies were obtained by housing female rats with male rats and examining vaginal smears daily during the cohabitation. The presence of a copulatory plug or sperm in the vaginal smear was designated Day 0 of pregnancy. Rats were sacrificed on Day 0, 4, 9, 11, 13, 15, 18, 20 of pregnancy as well as 3 days postpartum, and ovaries were quickly removed, frozen with liquid nitrogen and stored in –80°C freezer. Ovaries were collected from 3-4 rats for each time point. The University of Kansas Animal Care and Use Committee approved protocols for the care and use of rats used in the experiments.
Primers for regular PCR
TaqMan gene expression assays from Applied Biosystems
Catalog number and assay ID
Cat.#: 4331182, ID: Rn00567266_m1
Cat.#: 4331182, ID: Rn00562543_m1
Cat.#: 4331182, ID: Rn00570970_m1
Cat.#: 4331182, ID: Rn00592006_m1
Cat.#: 4351372, ID: Rn01450817_m1
Cat.#: 4331182, ID: Rn00564475_m1
Cat.#: 4331182, ID: Rn00570376_m1
Cat.#: 4331182, ID: Rn00585707_m1
Cat.#: 4331182, ID: Rn01416753_m1
Cat.#: 4331182, ID: Rn00667869_m1
Cat.#: 4331182, ID: Rn00566383_m1
Data points shown represent mean ± standard error. Statistically significant differences between data points of two groups were determined by Student’s t-test. By convention, a P value of < 0.05 was considered statistically significant.
In the present study, we have determined the relative expression levels of the nine proprotein convertase genes in rat ovaries throughout gestation. Of these nine proprotein convertase genes, only Pcsk1 and Pcsk6 were upregulated as gestation advanced, in parallel with the expression level of the relaxin gene. The others did not show a consistent pattern of change in the expression level. The results on the upregulation of Pcsk1 in rat ovaries during pregnancy agree with those of our previous study using porcine ovary . So far, the relative levels of other proprotein convertases in ovaries during pregnancy have not been reported.
It is difficult to ascribe a specific substrate for each of the proprotein convertases, because of their complementary and redundant functions. Since the levels of Pcsk1 and Pcsk6 transcripts were greatly increased in late pregnancy, these enzymes may be involved in the posttranslational processing of prorelaxin, the precursor of the major peptide hormone of the ovary, relaxin, in late pregnancy. Judging from the relative intensities of Pcsk1 and Pcsk6 transcripts (Figure 1), PACE4 (PCSK6) may play a more important role in the posttranslational processing of prorelaxin. Although the relative level of Pcsk1 expression increased over 15-fold at Day 20 of pregnancy while that of Pcsk6 increased only 4-fold (Figures 2 and 3), Pcsk6 is expressed at a much higher basal level than Pcsk1 (Figure 1). Therefore, PCSK6 will be present at a much higher level in the ovary than PCSK1. Whether it plays a more important role than PCSK1 remains to be verified experimentally. It is of great interest to study the posttranslational processing of prorelaxin, since its expression level in the ovary gradually increases during the course of pregnancy. First, results from our laboratory and others showed that prorelaxin is biologically active [20–22]. Unlike the structurally similar proinsulin, proteolytic processing is not necessary for the biological activities of relaxin. Second, with the exception of human and primate prorelaxins [23–25], the paired basic residues required for recognition and cleavage by the first seven members of proprotein convertases are not present at the B-chain/C-peptide junction in prorelaxins of the other species. Processing of these prorelaxins at the B-chain/C-peptide junction will require other proteases. The B-chain/C-peptide junction of rat prorelaxin does not possess the recognition sequence of SKI-1/S1P and PCSK9. Therefore, they are unlikely to be involved in the processing of prorelaxin. The identity of the proteases that process the prorelaxin at the B-chain/C-peptide junction remains to be studied. Third, the processing of prorelaxin is very slow, the majority of relaxin present in rat serum or ovary of late pregnancy were high molecular weight forms. On Day 20 pregnancy, only 25% of relaxin in rat serum was the 6 kDa relaxin . Two forms of high molecular weight (18 and 16.5 kDa) relaxin have also been detected in rat ovaries on Day 20 pregnancy and the combined concentration of these two forms was more than 30 times higher than that of 6 kDa relaxin .
As mentioned in the introduction, PC5/6 may be involved in the processing of precursors of transformation growth factor and matrix metalloproteinase family , and that of inhibin . On the other hand, furin may be involved in the processing of matrix metalloproteinase 2 . PC4 is suggested to be the enzyme that processes pituitary adenylate cyclase-activating polypeptide .
In conclusion, all nine proprotein convertases are expressed in the rat ovary during gestation. Only PC1/3 and PACE4 were found to be upregulated as gestation advanced. They may play an important role in the processing of proproteins in the ovary during pregnancy. However, it is difficult to ascribe a specific substrate to these proprotein convertases.
Membrane-bound transcription factor peptidase, site 1 gene
Neural apoptosis-regulated convertase-1
Paired amino acid converting enzyme 4
Polymerase chain reaction
Proprotein convertase subtilisin/kexin
small interfering RNA
Transforming growth factor beta.
This work was supported by a Grant from Albert Einstein Society to SCMK.
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