Figure 3
Effect of treating granulosa cells in vitro with control medium, FSH (0.33 ng/ml), IGF (10 ng/ml) or FSH+IGF in combination with PD98059 (Erk inhibitor) and/or LY2924002 (Akt inhibitor) on cell number and the secretion of oestradiol, progesterone, inhibin-A activin-A and follistatin (N = 3 replicates with 6 wells included per treatment per replicate). Bars with no common superscript are different (P < 0.05) within each treatment group.