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Figure 4 | Journal of Ovarian Research

Figure 4

From: Aberrant STYK1 expression in ovarian cancer tissues and cell lines

Figure 4

STYK1 protein expression is unaffected by estradiol and G-1 treatment. Ovarian tumor cell lines were treated with vehicle, 5 × 10-8 M estradiol, 1 × 10-8 M BSA-conjugated estradiol (E2B), and 1 × 10-8 M G-1 for 4-18 h for 18 hours. Forty μg of protein lysate from the indicated cell lines were electrophoresed by 12.5% SDS PAGE and transferred to PVDF membrane. The blot was probed with anti-STYK1 antibody then stripped and re-probed with α-tubulin as a loading control. This is a representative blot from three independent experiments. * indicates a value that is significantly different compared to 5 × 10-8 M estradiol treatment (p < 0.001).

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