Time-course effect of LH on Akt phosphorylation in bovine theca cells. Theca cells were plated onto serum-coated dishes with serum-free medium for 36 h and then stimulated with LH (100 ng/ml) for the stated times. Cytosolic extracts (20 μg) were subjected to immunoblotting with anti-phosphorylated-Akt antibody and anti-total-Akt antibody. Representative images (Top) and densitometric data of phospho-Akt contents (Bottom), expressed as ratio of phospho-Akt to total-Akt, are shown. * denotes means that are significantly different from 0 h (P < 0.01). ** denotes means that are significantly different from 0 h (P < 0.001).