Figure 2

Effect of H89, SB203580 or U0126 on ERK1/2 phosphorylation in cumulus cells. For reference, the COC that were cultured without FSH and LH for 5 h value was set as 1 and the data presented as the fold strength. Values are mean +/- SEM of 3 replicates. *: The significant differences were observed as compared with that in COCs cultured with FSH and LH for 5 h. **: The significant differences were observed as compared with that in COCs cultured with FSH, LH, H89 and EGF for 5 h. ***: The significant differences were observed as compared with that in COCs cultured with FSH, LH SB230580 and EGF for 5 h. The respective value of protein levels of ERK1/2 phosphorylation were normalized according to those of β-ACTIN to evaluate arbitrary units of the relative abundance. FSH(-): COCs were cultured without FSH and LH for 5 h; FSH(+): COCs were cultured with FSH and LH for 5 h; EGF(-): COCs were cultured without EGF for 5 h; EGF(+): COCs were cultured with EGF for 5 h; +H89: COCs were cultured with H89 for 5 h; +SB: COCs were cultured with SB203580 for 5 h; +U0126: COCs were cultured with U0126 for 5 h