A. BMP-2 expression in normal and malignant primary cultures. RNA expression levels were normalized to that of the control RNA by real time RT-PCR assays. Relative fold change expression was the ratio of the first EOC sample to that of other samples. Values represent the mean +/- SEM of two experiments. B. BMP-2 secretion in culture media of NOSE or EOC cell primary cultures. Cell-free supernatants were collected and tested for BMP-2 concentration by ELISA. Values represent the mean of duplicate experiments. Significance as compared to NOSE samples was defined as p < 0.05 using a Student t-test. C. BMP-2 mRNA expression in primary cultures of EOC from solid tumor and ascites. Expression levels were quantified by real time RT-PCR and compared to the control RNA (Erk-1) Relative fold change expression was the ratio of the EOC from primary tumor sample to that of ascitic sample from the same patient. Values represent the mean of two experiments.