Figure 3

Gene expression induced by BMP-2. Cells were treated with 50 ng/ml BMP-2 in complete media or with indicated doses for 90 min. Where indicated, 0.5 ng/ml Noggin (N) was used. RNA was extracted, retro-transcribed and used for real-time PCR using specific primers for Id1, Smad6, Snail or Wnt5a. Relative fold change expression was the ratio of treated cells to that of non-treated cells. Values are the mean +/- SEM of duplicate wells from at least two independent experiments. * p < 0.05 (Student t-test).