Binding of murine Muc16 to the lectin ConA. (A) Concentrated MOVCAR-2 spent media was separated on a ConA affinity column. Fractions eluted with ConA buffer were combined in pools 1 and 2. Fractions eluted with 100, 250, and 500 mM α-Me-Man concentrations were combined in pools 3–5, respectively. (B) Pooled fractions were electrophoresed by SDS-PAGE and probed with 653F. The arrow indicates migration of 250 kDa molecular weight marker. Murine Muc16 was detected primarily in pools 3–5.