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Figure 1 | Journal of Ovarian Research

Figure 1

From: The prosurvival activity of ascites against TRAIL is associated with a shorter disease-free interval in patients with ovarian cancer

Figure 1

Effect of peritoneal fluids on TRAIL-induced cell death in CaOV3 cells. (a) CaOV3 cells were pre-incubated for 2 h with OVC509 and OVC361 ascites (10% v/v) obtained from women with advanced serous ovarian cancer and treated with TRAIL (10 ng/ml) for 48 h. Cell viability was measured by XTT assay. Data are shown as the percent cell viability relative to untreated (no TRAIL, no ascites) cells. Results are from three independent experiments done in triplicate and express as mean ± SEM. (b) TRAIL IC50 was determined by XTT assay and defined as the concentration of TRAIL required to kill 50% of CaOV3 cells in the presence or absence of a specific ascites. The prosurvival activity of ovarian cancer ascites and benign fluids was determined by their ability to increase TRAIL IC50 after 48 h compared to the TRAIL IC50 of CaOV3 cells not exposed to peritoneal fluids. A value of 1 indicates a neutral effect of ascites on TRAIL-induced cytoxicity.

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