Effect of ovarian cancer ascites on TRAIL-, cisplatin- and paclitaxel-induced cell death in CaOV3 cells. CaOV3 cells were pre-incubated for 2 h with various fluids (10% v/v) obtained from women with advanced ovarian cancer and treated with increasing concentrations of TRAIL for 48 h or with cisplatin or paclitaxel for 72 h. Cell viability was assessed by XTT assays. TRAIL, cisplatin and paclitaxel IC50 were determined in the presence of ascites and expressed as fold increased relative to IC50 in the absence of ascites. A value of 1 indicates a neutral effect of ascites on these drugs. Results are from three independent experiments done in triplicate.