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Figure 4 | Journal of Ovarian Research

Figure 4

From: The prosurvival activity of ascites against TRAIL is associated with a shorter disease-free interval in patients with ovarian cancer

Figure 4

Effect of ovarian cancer ascites on TRAIL-induced cell death in primary ovarian tumor samples. (A) Primary cultures ovarian tumor cells (samples 346, 327, 318) were pre-incubated for 2 h with OVC509 (10% v/v) and treated with increasing TRAIL concentrations for 48 h. Cell viability was measured by XTT assay. Data are shown as the percent cell viability relative to TRAIL and ascites untreated cells. Results are from three independent experiments done in triplicate and express as mean ± SEM. *, indicates P < 0,001. (b) TRAIL IC50 were determined in the presence of OVC346 or OVC509 ascites and expressed as fold increased relative to IC50 in the absence of ascites for 9 primary cultures of ovarian tumor cells. Cells were isolated either from ascites (A) or from tissues (T). A value of 1 indicates a neutral effect of ascites on TRAIL cytotoxicity.

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