Figure 5

Ovarian cancer ascites OVC346 and OVC509 were incubated with primary cultures from sample 346A for 90 min. Lysates were obtained and Western blot analysis was performed with phospho-Ser473 Akt (p-Akt) and Akt antibody (Akt). Densitometric quantification of phosphorylated Akt from three separate experiments normalized to total Akt. Data are expressed as Akt phosphorylation fold increased relative to 349A cells not treated with ascites.