Figure 5

Effect of T0901317 treatment on apoptotic gene and BAX protein expression. After a 24 hour treatment, cells were harvested for isolation of mRNA as detailed in the methods section. A significant induction of BAX and caspase gene induction was demonstrated, especially at the 30 μM dose. Upregulation of the anti-apoptotic Bcl-2 gene expression was demonstrated with the 30 μM concentration (A-C), *P < 0.05, **P < 0.001). CaOV3 cells were grown in media supplemented with 10% FBS for 24 hours in presence of vehicle (DMSO) or the indicated concentrations of T0901317 (5 μM to 50 μM). Whole-cell extract was obtained and 60 μg of protein was analyzed for BAX protein levels by Western blot analysis. β-actin expression was used as an endogenous control (D).