PI3K and Akt activation after CCL25 treatment. Cells were tested for their ability to phosphorylate PI3Kp85-tyrosines and Akt-Ser473 following treatment with or without CCL25, cisplatin, wortmannin, and/or PF-573,228. FACE assays quantified in situ total and phosphorylated protein levels before (0) or after (5 or 10 minutes) CCL25 stimulation in the presence of cisplatin and inhibitors. The ratio of active (phosphorylated) to total PI3K or Akt are presented ± SEM from 3 separate experiments performed in triplicate. Asterisks (*) indicated statistical differences (p < 0.01) between untreated and treated cells.