Expression and localization of Brn-3a in (a-b) ovarian cancer cell lines by fluorescent microscopy. The expression and localization of Brn-3a was evaluated by using rabbit polyclonal Brn-3a antibody by fluorescent microscopy as described in the Methods and Materials. Cytoplasmic and nuclear staining were visualized using secondary Alexa 590 fluorescent labeled (red) antibody and DAPI (blue). Anti-mouse β-actin staining (green) followed by Alexa 488 (green) fluorescent labeling was used as an internal control. Magnification-400; scale = 50 μm.