Figure 3

Expression and localization of LRG1 protein in ovarian cancer tissues and cell lines. A) 50 µg of total protein extract from ovarian cancer tissues (N = 7) and normal ovaries (N =5) were evaluated by Western blot for LRG1 protein expression. Kidney was used as a positive control tissue, as it contains an abundance of epithelial cells. B) LRG1 protein expression in 20 µg of total protein extract from ovarian cancer cell lines and immortalized NOSE cells. β-actin was used as the loading control. C) Left panel; silver stained polyacrylamide gel of LRG1 purified from human plasma, PNGase F, and purified LRG1 treated with PNGase F. Right panel; Western blot for LRG1 in protein extracts from cell lines with and without PNGase F treatment. D) Subcellular localization of LRG1 is shown by immunocytochemistry in ovarian cancer cell lines (OVCAR5, OVCAR433, OV2008, C-13, and SKOV3) and immortalized NOSE cell line (1816-575); 200X magnification, scale bar = 20 µm. FITC (green) = LRG1, DAPI (blue) = nucleus.