Effect of in vitro treatment of different doses of GnRH-Ag on progesterone synthesis and western blot analyses of LH-R and 3β-HSD proteins in the ovaries of mice. Progesterone synthesis by the ovary A) without LH and B) with LH. Western blot analysis of LH-R C) without LH and D) with LH. E) Western blot analysis of 3β-HSD without LH. Densitometric analyses of the blots are shown in bar graph (n = 3). Values are mean ± S.E.M. * Values are significantly different (P < 0.01) versus control.