Figure 1

Expression of IL-1α by immunofluorescence. Expression of IL-1α protein in primary endometrial cells (A and B), endometrial cell line RL-952 (C and D) and the different subtypes of epithelial ovarian cancer cell lines TOV-112D (endometrioid), TOV-21G (clear cell) and OV-90 (serous) (E and F; G and H; I and J; respectively). Note the marked intensity of IL-1α staining in both endometrial cells (B and D) and ovarian cancer cells (F, H and J). No immunofluorescence was observed in negative controls for endometrial cells (A and C) and ovarian cancer cells (E, G, and I) in the absence of primary antibody (objective × 100).