Figure 5
ALK3QD signalling induces an EMT-like morphology of OVCA429 cells. (A) Subconfluent monolayer cultures of 429T-A44 and 429T-A54 cells, and 429T control cells, were treated with Dox for 2 d, or left untreated (inset). At confluence, both 429T-A44 and 429T-A54 cells exhibited a greater proportion of refractile, spindle-shaped cells versus 429T control cells. (B) All cells were grown and treated as described above, but after 2 d a scratch wound was generated. Long cytoplasmic projections extend into the wound space in Dox-treated 429T-A44 and 429T-A54 cells as compared with 429T control cells. Photo images were captured at 100 × original magnification.