Skip to main content

Table 1 Summary on gene validation by RT-PCR in comparison with gene array results

From: Mural granulosa cell gene expression associated with oocyte developmental competence

Gene Primer sequence PCR product size (bp) Location on rat chromosome GenBank Access # Fold changes (NDC/PDC) by
      gene array RT-PCR
Lysyl oxidase RV:AGTCTCTGACA 129 18q11 NM_017061 2.8 2.86
(LOX) TCCGCCCTA C      
  FW:ACCTGGTACCC      
  GATCCCTA      
Glycoprotein-4- FW:AGATAAAGATG 186 5q22 NM_053287 -1.7 -1.04
Beta-galactosyltrans GGCGGCCGTTACT      
ferase 2 (GGBT2; RV:ACATGGTGTCT      
UDP-Gal) CCAGCCTGATTGA      
Nerve growth factor FW:AATGATGGGTT 175 Xq35 NM_053401 1.6 1.03
receptor associated GGGTGGAGATGGA      
protein 1 (Ngfrap1; RV:ACCGAAGTCAA      
Bex3; Nade) GGCATAAGGCAGA      
Protein disulfide FW:ATATGACCGAG 185 11q22 NM_001014125 1.8 1.86
isomerase- CTGTGACGCTGAA      
associated 5 (Pdia5) RV:ACATCTTTGGC      
  TCCAGGGTCTTCT      
Protein disulfide FW:ACCTTCTTTCT 182 Chromo- NM_001004442 1.8 1.04
isomerase- AGCGGTCAGTGCT   some 6    
associated 6 (Pdia6) RV:AGTGCACTTGC      
  TGCTTTCTTCCAC      
Myeloid ecotropic FW:TAGCCACCAAT 99 14q22 XM_223643 1.6 1.33
viral integration site 1 ATCATGAGGGCGT      
homolog (Meis1) RV:TGAGTCCCGTA      
  TCTTGTGCCAACT      
CD83 antigen FW:ATGTGCCTGAA 193 17p12 NM_001108410 1.7 1.6
  TACCACCTGGACA      
  RV:AGCCGCATGAA      
  ACATGAAGCTGAC      
Lysozyme (Lyz) FW:TATGAACGCTG 95 7q22 NM_012771 1.7 1.37
  TGAGTTCGCCAGA      
  RV:TGCTGAGCTAA      
  ACACACCCAGTCT      
Trinucleotide repeat FW:TGAAGTACCTC 176 1q36 NM_001107549 1.7 1.03
containing 6 (Tnrc6) CACGATTTCGCCA      
  RV:TGCTGTTCTGC      
  ACCTCTCCGTTAT      
Interleukin 13 FW:AAGTGAGAAGC 155 Xq12 NM_145789 1.4 1.16
receptor alpha 1 CTAGCCCTTTGGT      
(Il13ra1) RV:AGTTGGTGTCC      
  GGGCTTGTATTCT      
18S rRNA FW:CGCGGTTCTAT 219 Chromo- M11188 Housekeeping  
  TTTGTTGGT   some 3    
  RV:AGTCGGCATCG      
  TTTATGGTC