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Table 1 Summary on gene validation by RT-PCR in comparison with gene array results

From: Mural granulosa cell gene expression associated with oocyte developmental competence

Gene

Primer sequence

PCR product size (bp)

Location on rat chromosome

GenBank Access #

Fold changes (NDC/PDC) by

     

gene array

RT-PCR

Lysyl oxidase

RV:AGTCTCTGACA

129

18q11

NM_017061

2.8

2.86

(LOX)

TCCGCCCTA C

     
 

FW:ACCTGGTACCC

     
 

GATCCCTA

     

Glycoprotein-4-

FW:AGATAAAGATG

186

5q22

NM_053287

-1.7

-1.04

Beta-galactosyltrans

GGCGGCCGTTACT

     

ferase 2 (GGBT2;

RV:ACATGGTGTCT

     

UDP-Gal)

CCAGCCTGATTGA

     

Nerve growth factor

FW:AATGATGGGTT

175

Xq35

NM_053401

1.6

1.03

receptor associated

GGGTGGAGATGGA

     

protein 1 (Ngfrap1;

RV:ACCGAAGTCAA

     

Bex3; Nade)

GGCATAAGGCAGA

     

Protein disulfide

FW:ATATGACCGAG

185

11q22

NM_001014125

1.8

1.86

isomerase-

CTGTGACGCTGAA

     

associated 5 (Pdia5)

RV:ACATCTTTGGC

     
 

TCCAGGGTCTTCT

     

Protein disulfide

FW:ACCTTCTTTCT

182

Chromo-

NM_001004442

1.8

1.04

isomerase-

AGCGGTCAGTGCT

 

some 6

   

associated 6 (Pdia6)

RV:AGTGCACTTGC

     
 

TGCTTTCTTCCAC

     

Myeloid ecotropic

FW:TAGCCACCAAT

99

14q22

XM_223643

1.6

1.33

viral integration site 1

ATCATGAGGGCGT

     

homolog (Meis1)

RV:TGAGTCCCGTA

     
 

TCTTGTGCCAACT

     

CD83 antigen

FW:ATGTGCCTGAA

193

17p12

NM_001108410

1.7

1.6

 

TACCACCTGGACA

     
 

RV:AGCCGCATGAA

     
 

ACATGAAGCTGAC

     

Lysozyme (Lyz)

FW:TATGAACGCTG

95

7q22

NM_012771

1.7

1.37

 

TGAGTTCGCCAGA

     
 

RV:TGCTGAGCTAA

     
 

ACACACCCAGTCT

     

Trinucleotide repeat

FW:TGAAGTACCTC

176

1q36

NM_001107549

1.7

1.03

containing 6 (Tnrc6)

CACGATTTCGCCA

     
 

RV:TGCTGTTCTGC

     
 

ACCTCTCCGTTAT

     

Interleukin 13

FW:AAGTGAGAAGC

155

Xq12

NM_145789

1.4

1.16

receptor alpha 1

CTAGCCCTTTGGT

     

(Il13ra1)

RV:AGTTGGTGTCC

     
 

GGGCTTGTATTCT

     

18S rRNA

FW:CGCGGTTCTAT

219

Chromo-

M11188

Housekeeping

 
 

TTTGTTGGT

 

some 3

   
 

RV:AGTCGGCATCG

     
 

TTTATGGTC

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