Figure 3
Comparison of the expression of mesothelin protein in mRNA positive tumor ovaries (left), mRNA negative tumor ovaries (middle) and in normal ovaries (right). Mesothelin was detected at a pI range of 5.6 to 6.6 and molecular weights of 80 and 40 kDa (solid circles) in 2D Western blots using a monoclonal antibody to human mesothelin (clone 4H3). No mesothelin was detected in mRNA negative tumor ovaries or in normal ovaries. Some faint reactions (dotted circle) were observed in mRNA positive tumor ovaries blot and the identity of those spots remains to be determined.