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Table 1 RT-PCR primer pairs used for mesothelin and actin transcript amplification

From: The hen model of human ovarian cancer develops anti-mesothelin autoantibodies in response to mesothelin expressing tumors

amplification target primer sequence, 5' - 3' expected product size
Amplicon1 For -GGCAAAGCTAGGGAGCTTG
Rev-AGGCCCAAACACAGTGTTG
772 bp
Amplicon2 For-ACCGCAGAGGATGTTAGCAA
Rev-TGTGAACAGGCTGAAGGATG
251 bp
Amplicon3 For-GATGCTTTAATGAGCCTGACG
Rev-GCTGAAACTTCGGCGTGAC
202 bp
actin For-GCCCTCTTCCAGCCATCTTT
Rev-TGGAGTTGAAGGTAGTTTCATGGAT
67 bp