Table 1 RT-PCR primer pairs used for mesothelin and actin transcript amplification
amplification target | primer sequence, 5' - 3' | expected product size |
|---|---|---|
Amplicon1 | For -GGCAAAGCTAGGGAGCTTG Rev-AGGCCCAAACACAGTGTTG | 772 bp |
Amplicon2 | For-ACCGCAGAGGATGTTAGCAA Rev-TGTGAACAGGCTGAAGGATG | 251 bp |
Amplicon3 | For-GATGCTTTAATGAGCCTGACG Rev-GCTGAAACTTCGGCGTGAC | 202 bp |
actin | For-GCCCTCTTCCAGCCATCTTT Rev-TGGAGTTGAAGGTAGTTTCATGGAT | 67 bp |