Autofluorescent necrotic malignant tissue with clearly visible hypertrophic primitive oocyte-like cells (arrows) releasing from their "chambers". (A) Stained for the expression of SSEA-4, marker (green) of pluripotency. (B) Stained for the expression of SOX-2, marker (red) of pluripotency. (C) After DAPI (blue) staining. (D) Non-stained. (fluorescence and light microscopy, magnifications 400×). Legend: h-hypertrophic/necrotic cells.