Figure 1
Establishment of drug resistant cell lines and gene expression profiling. A. IC50 values for the various cell lines used in this study. Thick outlined squares show resistance levels for the drug against which the corresponding cell lines were derived. White squares denote lack of resistance, and light gray squares, moderate resistance. Dark gray indicates drug resistance over 10-fold compared to the parental OV90 line. B. Multi-dimensional scaling plot indicating the cell lines used for the gene expression profiling analysis. Each of the two different resistant clones obtained from the 3 different drugs were cultured and analyzed in duplicate. Two cultures were analyzed for the parental OV90 (OV90-1 and OV90-2). C. Venn diagram representing the number of genes significantly altered in each type of drug resistance. A total of 68 genes were found altered in all three types of resistance generated following exposure to cisplatin, doxorubicin, and paclitaxel.