Figure 1

Expression of H- Pgds in the mouse adult ovary. (A), In situ hybridization for H-Pgds and granulosa cell marker FoxL2 was performed on sections from wild type adult ovaries. Primary, secondary, pre-antral, antral, ovulatory follicles and corpus luteum are represented for H-Pgds and FoxL2 mRNA expression and expressing granulosa cells (GC) are labeled by a blue arrow. Scale bars = 50 μm. (B), H-Pgds protein expression was detected in granulosa cells on wild type adult ovary sections, using an anti-H-Pgds antibody (in green) whereas nuclei are labeled in blue by the Hoescht Dye (HST). The merge panel has been enlarged on the right bottom panel. Arrows indicate H-Pgds expressing granulosa cells. TC, theca cells; GC, granulosa cells. Scale bar = 50 μm. (C), Control immunofluorescence experiment with no primary H-Pgds antibody (IgG control) showing the specificity of the antibody. AMH staining in granulosa cells was used on the same slide. Arrows indicate granulosa cells (GC).