Figure 6

PGD2 signaling is necessary for FSH action. Adult cycling female mice were treated with 5 I.U. PMSG without (PMSG) or with (PMSG+HQL-79) administration of HQL-79 inhibitor. FshR, LhR (A), Sf-1 (B), Cyp11A1 (C) and StAR (D) gene expression levels in ovaries (n = 5 for each condition), were analyzed by real-time RT-PCR. The values of at least two repeats of two different RT reactions were averaged and normalized to Gapdh expression. Values represent mean +/- SE and * represents significant differences P < 0.05 (A), P < 0.001 (C-D) compared with ovaries treated with PMSG only.