S1P1 mRNA expression in hen tissues. (A) S1P1 mRNA (226 bp) is expressed in both normal and tumor ovaries. Examples of mRNA in tumors with endometrioid (En), serous (Sr), and mucinous (Mc) histology are shown. (B) Examples of other hen tissues that express S1P1 mRNA (226 bp) include liver (LVR), kidney (KDNY), skeletal muscle (MSCL), oviduct (OVDT) and spleen. Normal ovary and spleen are from the same hens (1-4). β-actin (300 bp) was used as a loading control. Controls for S1P1 primer include the positive control (+) lane which was the sample from an earlier experiment used to verify the RNA sequence. The negative control lane omitted the cDNA.