Figure 3
Colony formation in MUC4-trasfected cells. SKOV3-Vector and SKOV3-MUC4 cells were seeded in equal confluence and allowed to grow for up to 18 days. After the full confluence we observed a tumor sphere-like colony formation on the top of the cells. Circular colony formation was observed only in MUC4 overexpressed SKOV3 cells and no colonies were formed in SKOV3-vector cells. (Original magnification 100X upper panel and 40X lower panel).