Figure 4
Expression of cancer stem cell markers in circular colonies. (A) Confocal analysis showed significant expression of CD133 (Red) in isolated colonies compared to SKOV3-MUC4 cells. MUC4 (Green) expression was seen in both isolated colonies and SKOV3-MUC4 cells. DAPI (Blue) was used as nuclear counter staining. (B) Western blot analysis showed MUC4, HER2, ALDH1, CD133 and Shh expression in SKOV3-MUC4 and isolated colonies from MUC4 overexpressed SKOV3 cells. β-actin served as a loading control. DIC - differential interference contrast and staining (Scale bar-20 μm).