In vitro granulosa cell culture progesterone levels following exposure to metformin during seven days of metformin treatment. Experiments were performed with 5 mice per genotype. Granulosa cells were pooled, divided into groups by metformin concentration and duration of culture, and media was collected for analysis. Experiments were performed in triplicate. Error bars are SEM. Compared to respective controls, no difference (P > 0.05, t test) was observed in progesterone levels of media in any metformin concentration during any time point in any genotype.