Individual cell death effects of metformin and PEITC. A. Metformin treatment [8 mM] on multiple human ovarian cancer cell lines after 48 hours shows minimal cell death induction. B. PEITC [5 μM] induces significant cell death of multiple human ovarian cancer cell lines after a 24 hour treatment. Cell death percentage was determined using hemacytometry with Trypan blue exclusion to count both live and dead cells. * p < 0.01.