Figure 3

Immunodetection of autopahgy proteins in ovarian cancer tissue sections. Immunofluorescence positivity for beclin 1 (panel A) and LC3 (panel B) appears as discrete puncta (indicative of protein clusterization) in the cytoplasm of cancer cells. A high level of expression of beclin 1 and of LC3 (positivity in > 40% of cancer cells) was found to correlate with good prognosis (CR, complete remission at 5 years of follow up). Immunohistochemistry of beclin 1 in a positive (panel C) and in a negative (panel D) case is also shown (magnification 420x). The presence or absence of beclin 1 aggregates can be appreciated in the high-magnification (1050x) area shown in the insets.