Q-PCR analysis of various gene transcripts in ovaries from control, 2D and 7D after PMSG treated mice. Note an increase in Fshr mRNA expression after PMSG treatment on D2, which slightly reduced by 7D of PMSG treatment. All other markers specific for pluripotent stem cells, germ cells, oocytes and meiosis showed an increase in mRNA expression after PMSG treatment to varying degree except for HoxA10. Increase in pluripotent (Oct-4A and Nanog) and meiotic marker (Scp3) after PMSG treatment suggest that the VSELs get stimulated by PMSG treatment, undergo further proliferation, differentiation and meiosis leading to neo-oogenesis and PF assembly. Each sample was obtained by pooling ovaries from two animals.