Figure 7
Follicle stimulating hormone receptor (FSHR) immunolocalization in estrus stage and after 7D of PMSG treatment. A &B In control, minimal staining is observed in the OSE cells. However, distinct immunolocalization is observed in the oocyte of the PF (black arrow), whereas the surrounding granulosa cells are negative. The granulosa cells in large follicles show positive staining (blue arrow). Positive staining is also observed in the oocyte in large follicle (asterisk). C &D Increased immunostaining pattern is observed after PMSG treatment in the multilayered OSE, oocytes of primordial and developing primary to secondary follicles. Inserts show follicles in various developmental stages with granulosa cells negative for FSHR. Note change in shape of the granulosa cells from flat to cuboidal as the PF transits into a primary follicle. Bar=20μm.