Stage | Total number of animals | Number of ovaries |
---|
Total | Histology and IHC
§
| RNA isolation
#
|
---|
Estrous | 2 | 4 | 4 | - |
Pro-estrus | 1 | 2 | 2 | - |
Di-estrus | 10*
| 20 | 8 | 12 |
2D PMSG | 12*
| 24 | 14 | 10 |
7D PMSG | 12*
| 24 | 14 | 10 |
- * Total number of animals used in 4 different experiments.
-
§ Whole ovary was sectioned and every third slide was used for H&E staining. The rest of the slides were used for IHC.
- #Two ovaries from two animals were pooled to make one sample for RNA isolation. Only samples showing good A260/A280 ratio after RNA isolation were used for RT/Q-PCR study.