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Table 1 Experimental design of the study

From: Gonadotropin treatment augments postnatal oogenesis and primordial follicle assembly in adult mouse ovaries?

Stage

Total number of animals

Number of ovaries

Total

Histology and IHC §

RNA isolation #

Estrous

2

4

4

-

Pro-estrus

1

2

2

-

Di-estrus

10*

20

8

12

2D PMSG

12*

24

14

10

7D PMSG

12*

24

14

10

  1. * Total number of animals used in 4 different experiments.
  2. § Whole ovary was sectioned and every third slide was used for H&E staining. The rest of the slides were used for IHC.
  3. #Two ovaries from two animals were pooled to make one sample for RNA isolation. Only samples showing good A260/A280 ratio after RNA isolation were used for RT/Q-PCR study.