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Table 1 Experimental design of the study

From: Gonadotropin treatment augments postnatal oogenesis and primordial follicle assembly in adult mouse ovaries?

Stage Total number of animals Number of ovaries
Total Histology and IHC § RNA isolation #
Estrous 2 4 4 -
Pro-estrus 1 2 2 -
Di-estrus 10* 20 8 12
2D PMSG 12* 24 14 10
7D PMSG 12* 24 14 10
  1. * Total number of animals used in 4 different experiments.
  2. § Whole ovary was sectioned and every third slide was used for H&E staining. The rest of the slides were used for IHC.
  3. #Two ovaries from two animals were pooled to make one sample for RNA isolation. Only samples showing good A260/A280 ratio after RNA isolation were used for RT/Q-PCR study.