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Table 2 Details of antibodies used for immunolocalization studies

From: Gonadotropin treatment augments postnatal oogenesis and primordial follicle assembly in adult mouse ovaries?

Antibody

Marker

Source

Dilution

Reason to conduct IHC

Ref

FSHR Polyclonal

FSHR: membrane bound receptor of FSH

Gift

1:100

To study cells responsive to PMSG

[49]

PCNA Monoclonal

PCNA: nuclear protein that functions during DNA replication

Sigma

1:5000

To study proliferating cells

[50]

OCT-4 Polyclonal

OCT-4 isoforms: OCT-4A is a nuclear transcription factor and OCT-4B is cytoplasmic

Abcam

1:100

To study pluripotent and progenitor stem cells

[32]

SCP3 Polyclonal

SCP3: a nuclear protein required for homologous recombination during meiosis I

Abcam

1:500

To study presence of cells undergoing meiosis

[36]

VASA Polyclonal

MVH: germ cell specific cytoplasmic protein expressed from primordial germ cell stage

R&D System

1:500

To study early germ cells and oocytes

[51, 52]