The proliferation of the Caov-3 cells following stimulation with G1, a selective GPR30 agonist, and G15, a selective GPR30 antagonist. Caov-3 cells were transfected with the pcDNA3.1 vector (Vector alone) or pcDNA3.1-GPR30 (GPR30). (A) The mRNA expression of GPR30 in Caov-3 cells was confirmed by semiquantitative RT-PCR. (B) The proliferation of the Ca-ov3 cells transfected with pcDNA3.1-GPR30 was examined using the MTS assay. The Caov-3 cells transfected with pcDNA3.1-GPR30 were treated with G1 (100 nM), G15 (100 nM), or both G1 and G15 (100 nM each) for 48 hours. The cell number is expressed as a percentage of the control (100%) which Caov-3 cells transfected with pcDNA3.1-GPR30. The values shown represent the means ±SE of three independent experiments performed in quadruplicate in three different passages of the cell lines. Significant differences are shown by asterisks: **, p<;0.01.