G1, a selective agonist of GPR30, induced the phosphorylation of the EGFR and Akt. (A) After treatment with or without 10 nM of EGF for 5 min or 100 nM of G1 for 5 min, cells were harvested and used to prepare cell lysates. The lysates were subjected to SDS-PAGE and blotted with anti-phospho EGFR (upper panel) or anti-EGFR (lower panel) antibodies. (B) Cells were treated with or without 10 nM of EGF for 5 min, 100 nM of G1 for 5 min, or 10 nM of PP1, followed by G1 treatment. Cell lysates were subjected to SDS-PAGE and blotted with anti-phospho Akt (upper panel) or anti-Akt (lower panel) antibodies. The values shown represent the means ± standard deviation from at least three separate experiments. Significant differences are indicated by asterisks. **; p<;0.01 (Additional file 2: Supplemental data 1).