Figure 4
Expression of mTOR and serine 2448–phosphorylated form of mTOR (P-mTOR) double staining by immunohistochemistry in DHEA-treated mouse ovary. mTOR expression was shown mainly cytoplasmic nature of total within granulosa cells in various stages of follicular development in control, DHEA-treated and vehicle groups. P-mTOR expression was highly enriched in mitotic granulosa cells in all stage of developing follicles in three groups (arrow, A–D). Negative control has no staining (D, insert).