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Table 2 Validation gene set, genes with significant changes in one or more of the resistant cell lines according to microarray analysis

From: Distinct genetic alterations occur in ovarian tumor cells selected for combined resistance to carboplatin and docetaxel

Cell line Gene designation Protein name General function Fold change P value for fold change Fluorescence value resistant line Fluorescence value parent line
A2780CBN AKR1C3 Aldo-keto reductase family 1 member C3 Catalyzes the conversion of aldehydes and ketones to alcohols −3.07 4.94 x 10-3 18.75 56.25
  ANXA1 Annexin A1 Calcium dependent phospholipid binding protein −104.15 1.38 x 10-4 96.50 9992.50
  CDH7 Cadherin 7 Cell to cell adhesion glycoprotein 2.08 1.29 x 10-4 67.25 32.75
  GCLC Glutamate-cysteine ligase catalytic subunit Rate limiting enzyme of glutathione synthesis 8.50 5.85 x 10-5 6907.25 815.00
  GSTO1 Glutathione S-transferase omega 1 Stress response protein, catalyzes addition of glutathione to toxic substrates 2.38 1.07 x 10-3 23166.50 9726.50
  MT2A Metallothionein 2A Heavy metal binding protein 3.69 4.15 x 10-6 4899.50 1266.00
  PARP9 Poly(ADP)-ribose polymerase family, member 9 Catalyzes addition of ADP-ribose moieties to substrate proteins 9.40 1.95 x 10-3 2523.25 275.25
A2780DXL CYP1B1 Cytochrome P450 family 1, subfamily B, polypeptide 1 Phase 1 enzyme in drug metabolism −37.77 2.00 x 10-8 491.50 14858.38
  LGI1 Leucine-rich glioma inactivated 1 Metastasis regulator 175.85 1.03 x 10-9 5449.25 30.00
  ABCB1 ATP-binding cassette transport subfamily B, member 1 Multidrug transporter (MDR/TAP family) 33.62 8.22 x 10-11 21065.40 649.60
  ABCB4 ATP-binding cassette transport subfamily B, member 4 Multidrug transporter (MDR/TAP family) 141.27 5.88 x 10-10 11876.75 84.63
A2780CBNDXL ABCB1 ATP-binding cassette transport subfamily B, member 1 Multidrug transporter (MDR/TAP family) 35.28 2.43E-03 474.25 13.00
  ABCB4 ATP-binding cassette transport subfamily B, member 4 Multidrug transporter (MDR/TAP family) 22.70 1.89x10-03 108.75 5.00
  AKR1C3 Aldo-keto reductase family 1 member C3 Catalyzes the conversion of aldehydes and ketones to alcohols 7.30 2.62 x 10-3 400.75 55.75
  FLRT3 Fibronectin Leucine-rich Repeat Transmembrane protein 3 Helps regulate cadherin mediated cell adhesion and cell morphogenesis −115.40 1.79 x 10-3 39.50 4423.25
  GSTO2 Glutathione S-transferase 2 Stress response protein, catalyzes addition of glutathione to toxic substrates 5.34 2.68 x 10-4 2133.25 400.00
  CDH7 Cadherin 7 Cell to cell adhesion glycoprotein 68.82 9.24 x 10-4 5223.75 75.70
  CDH11 Cadherin 11 Cell to cell adhesion glycoprotein 1022.24 1.57 x 10-4 10708.75 10.50