Figure 1
From: Discovery of dachshund 2 protein as a novel biomarker of poor prognosis in epithelial ovarian cancer
Expression of DACH2 in the cisplatin-sensitive A2780 ovarian cancer cell line compared to the cisplatin-resistant cell line A2780-Cp70 and validation of the specificity of the DACH2 antibody in A2780 ovarian cancer cells. Substantially higher DACH2 protein expression was seen in the cisplatin-resistant A2780-Cp70 cell line compared to its parental cisplatin-sensitive A2780 cell line by A) immunocytochemical staining and (B) immunoblotting, showing a major band at 62 kDa corresponding to the expected molecular weight of the DACH2 protein. (C) Relative mRNA expression was also higher in the A2780-Cp70 cells compared to A2780 cells as shown by qRT-PCR analysis. Data shown are mean ± SD of a representative experiment of two independent experiments performed in triplicate. (D) DACH2 protein expression was significantly decreased after transfection with siRNA against DACH2 in A2780-Cp70 cells as shown by immunocytochemistry 72 hrs post-transfection.