Validation of IL-1β expression and cell adhesion assay. A. RT-PCR analysis of IL-1beta was performed on FOC3 and MFOC3 mRNA using GAPDH as the internal control. B. Expression of IL-1β protein. Western blot analysis was performed on FOC3 and MFOC3 cell samples with anti- IL-1β, using β-actin as the loading control. C. Tumor cell adhesion assay. GFP-labeled FOC3 (▲) and MFOC3 (●) cells were added to the confluent mesothelial cell monolayer for 10, 20, 30 and 60 minutes and counted using fluorescent microscopy after washing away unattached cells. Each point represents the mean ± SD of at least three times separate experiments. * p < 0.05, ** p < 0.01.