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Figure 1 | Journal of Ovarian Research

Figure 1

From: Insulin and insulin-like growth factor signaling increases proliferation and hyperplasia of the ovarian surface epithelium and decreases follicular integrity through upregulation of the PI3-kinase pathway

Figure 1

Insulin and IGF-I induce OSE hyperplasia and proliferation. Ovarian organoids were cultured in alginate hydrogels in basal media (A) or media supplemented with 5 μg/ml insulin (B) or IGF-I for 7d (C). BrdU was added 24h prior to fixation to label dividing cells. Tissues were stained with hematoxylin and eosin (H&E) to evaluate morphology. Arrow indicates primordial follicle. Dashed line is representative of the field of OSE expansion. Alginate hydrogel stains lightly with hematoxylin and is marked by G. Serial sections were stained with antibodies against cytokeratin 8 (CK8) to mark OSE or antibodies against BrdU to mark dividing cells. All images are 400X.

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Journal of Ovarian Research

ISSN: 1757-2215

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