Figure 2
Histology of marmoset ovarian cortical tissue: (A) OSE and PF were visualized in cortical tissue sections after Haematoxylin & Eosin staining prior to culture. (B &C) Three days culture in untreated control showed minimal OSE, few PF with pycnotic nuclei and disorganized cortex (D &E) Ovarian tissue sections after FSH treatment showed OSE proliferation and PF growth and transition to primary and secondary stage as evident by change in shape of the surrounding granulosa cells from flat and single layered in untreated control (A) to cuboidal and 2-3 layers after growth (E). (F &G) Ovarian tissue sections after bFGF treatment showed OSE proliferation and PF growth and transition to primary and secondary stage. Inset in F shows a darkly stained stem cell amongst OSE cells. Scale bar = 20 μm.