Histology of human ovarian cortical tissue. (A-D) Young ovarian tissue sections and (E- G) Peri-menopausal ovarian tissue sections (A) OSE was not visualized but PF were seen in the cortex prior to culture (B) Untreated group revealed disorganized stroma with distorted PF. Healthy primary follicles were observed in the cortex after three days culture with (C) FSH and (D) bFGF treatment. (E) Prominent single layer of OSE and stroma devoid of follicles in peri-menopausal ovarian tissue (F) Loss of epithelial cells and disorganized stroma were evident after three days culture without any treatment (G) FSH treatment resulted in proliferation and multi-layered appearance of OSE. Scale bar = 20 μm.