Figure 2

Melting profiles of selected high-grade serous ovarian cancer samples obtained from MS-HRM experiments for PALB2 methylation analysis. The melting profiles were partly smoothened by applying a light digital filter available in the Rotor-Gene 6000 analysis software. Melting profiles for fully methylated (100%), 50%, 10% and unmethylated (0%) standards as well as the tumour samples are shown as red, turquoise, black, green and blue curves. T m plots (negative first derivative of the melting curves) are shown in the left panel and the corresponding difference plots are shown in the right panel, respectively. In difference plots, the melting profiles of the fully methylated standard are chosen as a baseline and the relative differences in melting profiles of all other samples are plotted relative to this baseline. The high-grade serous ovarian cancer DNAs (blue curves) are unmethylated for PALB2 as can be seen by their superimposition on the unmethylated standard (green curves).