Figure 1

SFN mediates the survival of PA-1 cells, and thus inhibits proliferation: (A) In vitro study was initiated by treating each of the PA-1 cells with increasing doses of SFN for 24 to 72 hours. The survival of these SFN-treated cancer cells was then measured using the MTT method; (B) Influence of SFN on apoptosis in PA-1 cells; (C) Total apoptosis in PA-1 cells after 4 h of incubation with SFN; (D) Caspase-3 activation in PA-1 cells following SFN treatment. After being treated with SFN, cells underwent western blot analysis and band intensities (pro-caspase 3) were quantified using an Li-COR near infrared imaging system. Statistical analysis was performed using a t-test; the symbols *, & and # in each group of bars indicate that the differences resulting from treatment with SFN are statistically significant at P < 0.05.