Figure 3

Unsupervised classification of 39 differentially expressed EMT associated genes (61 probe sets) demonstrates significant divergence between most primary and metastatic samples. Unsupervised hierarchical clustering of EMT associated genes differentially expressed across all samples demonstrates that the primary and metastatic samples of only one patient (620) are clustered most closely with one another. Primary and metastatic samples of all other patients cluster away from one another consistent with a model whereby all of the metastatic samples have undergone EMT while displaying a range of partial or complete (patient 620) compensating MET transitions at the metastatic site. The alternative hypothesis that metastasis occurs in the absence of EMT is definitively consistent with the molecular profiles of only one Group 1 patient (620).