Figure 3
Immunohistochemical expression of CD163 in ovarian tissues. Frozen sections were stained by the immunoperoxidase method as described in Materials and Methods. Representative fields were photographed at 200 X magnification. (a) Normal ovarian tissue; (b) benign ovarian tissue; (c) borderline ovarian cancer tissue; (d) grade 1 ovarian tumour; (e) grade 2 ovarian tumour; (f) grade 3 ovarian tumour. Black arrows denote brown CD163 staining in non-epithelial cells. Red arrows indicate unstained epithelial cells. Negative controls from (g) normal and (h) grade 1 tumour. (i) Tissue staining was quantified using Leica QWin Image Analysis Software, determining ratio of stain/whole tissue. Statistically significant results compared with control samples are denoted with * (Mann–Whitney test, P<0.05).